RNA

Part:BBa_K4329002

Designed by: Yuxin He   Group: iGEM22_BS_United_China   (2022-09-24)


sgRNA for targeting Bifunctional BirA enzyme

What is it?

CRISPR/Cas9 is a gene editing technology used to inhibit the expression from the targeted sequence inducing DNA to activate its self-repair process.

Usage and Biology

This system insures the maximum yield of biotin. We have designed a sgRNA sequence (AAGTCACCATACGTTAGTAC) targeting the Bifunctional BirA enzyme after the production of biotin to prevent biotin from further disintegration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 19
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None